P22-16 : Change in rhizosphere microbial community of Miscanthus condensatus, a pioneer plant on recent Miyake-jima volcanic deposit, during the vegetation development
Posted On 20 10月 2014
1Ibaraki Univ. College of Agri. , 2National Research Instit. for Cultural Properties Tokyo, 3Faculty of Life and Environ. Sci., Univ. of Tsukuba
Whereas microbes firstly colonize the new volcanic deposits and drive the initial geochemical cycle, the role of pioneer plants is of big significance as they enhance carbon input into soil and directly influence soil microbiome. However, the influence of pioneer plant on the rhizosphere community during the vegetation development is still unclear. In this study, we analyzed the rhizosphere communities of Miscanthas condensatus, colonizing the recent volcanic deposits in the Island of Miyake. Plants with rhizosphere soil were collected from four sites with different colonization levels of M. condensatus colonization: few (OY), sparsely (IG1), fully (IG2) colonized sites, and fully colonized site with partial shrub (IG3). Total DNA of rhizospheric community was subjected to T-RFLP profiling and clone library analyses targeting bacterial 16S rRNA and fungal ITS rRNA genes. Overall, bacteria of Acidobacteria, Actinobacteria, and Alphaproteobacteria were higher in the fully colonized sites (IG2 and IG3), while Betaproteobacteria and Gammaproteobacteria were higher in the few and sparsely colonized sites (OY and IG1). Ascomycota and Basidomycota dominated the fungal communities at the four sites. These results suggest that the influence of pioneer plant on the bacterial community in rhizosphere is higher than those on the fungal community during the vegetation development.
keywords:rhizosphere,pioneer plant,vegetation development,early soil ecosystem,