1Dept. Biochem., Fac. Biotechnol. Biomol. Sci., Putra Univ., 2Dept. Appl. Chem. Biochem. Eng., Fac. Eng., Shizuoka Univ., 3Dept. Biomol. Sci., Fac. Appl. Sci., MARA Univ. Tech.
At present, the bioremediation technique involving bacteria have been the target of phenol remediation technologies. The efficiency of phenol biodegradation can be enhanced by a process of cell immobilisation. From the 115 samples collected from different locations, 37 pure phenol-degrading bacteria were isolated of which 6 were able to degrade 100% 500 mg / L phenol. From the 6 isolates, bacterial Isolate number SA28s (i) isolated from Johor, has the best capability to degrade phenol in a mineral salt medium, pH 7.5 at 30 ° C, after 4 days of Incubation Compared with the Other Isolates. Isolate SA28a (I) Tentatively named it as Acinetobacter sp. strain AQ5NOL 1 USING molecular Phylogenetics Analysis of the 16S rRNA Gene Sequenced. Studies Were Carried out to Optimise the Degradation of phenol and Bacterial growth by free and immobilised cells in gellan gum. The combination of 0.04% (w / v) ammonium sulphate and 0.01% (w / v) of NaCl at pH 7 (phosphate buffer) gave optimum degradation of phenol and bacterial growth by the free cells . The Combination of 0.75% (v / w) gellan Gum, 300 beads, and Bead size of 3 mm Gave Optimum phenol Degradation by the Immobilised cells. Acinetobacter sp. strain AQ5NOL 1 Immobilised in gellan Gum beads Showed Enhanced Degradation of elevated Concentrations of phenol (1900 mg / L) compared to the free cells (1100 mg / L) and could be reused for at least 45 cycles.