Effect of nanobubbles on growth and respiration of cyanobacteria
1Dept. of Biological Sciences, Chuo Univ.
Nanobubbles that have diameters smaller than one micrometer are expected to be applied for many different field of biotechnology. We could generate very small air-nanobubbles, smaller than 100 nm, in water using a hand mixer. The bubbles were stable in water containing 1% ethanol more than one month. The zeta potential of the bubbles was between -30 to -40 mV. The addition of NaCl to nanobubble-water made the bubbles larger in size and lesser in numbers. When a cyanobacterium, Anabaena sp. PCC7120 was grown in the culture medium containing air-nanobubbles, it showed better growth than that in usual one. Dark respiration of the cyanobacterial cells was enhanced by air-nanobubbles. Nanobubbles may somehow affect physiology of microorganisms.