• 過去の学会大会
    • 環境微生物系合同大会2014
  • 微生物生態学会(JSME)のページへ
  • Main
    • 大会情報
  • for participants
    • 参加者の方へ (Participants)
    • 参加登録・講演要旨登録 (Registration, Abstract Submission)
    • 発表にあたっての注意事項 (Instruction for presentation)
  • Map&access
  • Program
  • abstract
    • 2015 KeyNote&Plenary
    • 2015JTKsymposium
      • IMS
      • IYS
    • 2015JSMEsymposium
      • JS1
      • JS2
      • JS3
      • JS4
      • JS5
      • JS6
      • JS7
      • JS8
      • JS9
      • JS10
      • JS11
      • JS12
      • JS13
      • JS14
      • JS15
      • JS16
      • JS17
      • JS18
      • JS19
      • JS20
      • JS21
      • JS22
    • 2015Oral
      • OA
      • OB
      • OE
      • OF
      • OH
      • OI
      • OJ
      • OK
      • ON
    • 2015Poster
      • PA
      • PB
      • PC
      • PD
      • PE
      • PF
      • PG
      • PH
      • PI
      • PJ
      • PK
      • PL
      • PM
      • PN
      • PO
      • PJ
      • PK
      • PL
      • PM
      • PN
      • PO
    • 2015ResearchWorkingGroup
    • 2015HighSchool
  • 7th JTK
    • Access&Maps
  • お問い合わせ
    • 展示会・広告のご案内

PB-027:

Who produces N2O in cropland fields?

Posted On 06 10月 2015
By :
Comment: Off
Tag: MC: 物質循環(material cycles), SE: 土壌生態系(soil ecosystem)

Isobe, Kazuo1, Wei, Wei1, Shiratori, Yutaka2, Nishizawa, Tomoyasu3, Otsuka, Shigeto1, Senoo, Keishi1
1The University of Tokyo, 2Niigata Agricultural Research Institute, 3Ibaraki University

Agricultural fields are one of the largest anthropogenic sources of atmospheric N2O. A wide phylogenetic range of microbes including bacteria, archaea and fungi are involved in N2O production through nitrification and denitrification. However, most of the previous studies on the microbial N2O production in agricultural fields have focused on the proteobacterial denitrifiers and nitrifiers. We have recently developed the molecular technique which allows the detection of the phylogenetically-diverse denitrifiers including non-proteobacteria, archaea and fungi. Thus, the objective of this study is to revise the microbial ecology of the N2O emission in agricultural fields.

The study field is a cropland located in Niigata, Japan. N2O flux in the field after fertilizations of organic matter or urea was measured every week during the cultivation period. Total RNA was extracted from the soil and marker genes for the production and reduction of N2O (i.e. proteobacterial and non-proteobacterial nirK and nirS, fungal nirK, bacterial and archaeal amoA, and bacterial nosZ) were quantified using RT-qPCR to estimate the contribution of each microbial functional group to the N2O emission.

Two N2O flux peaks were observed after the moderate and heavy rainfalls in the field amended with the organic fertilizer. The transcript abundances of the previously unaccounted bacterial nirK, bacterial and archaeal amoA, and bacterial nosZ increased at the first peak and the abundance of the fungal nirK increased at the second peak. On the other hand, only one N2O flux peak was observed after the heavy rainfall in the field with the urea fertilizer. Then, the transcript abundances of the fungal nirK and bacterial amoA increased. These results show that more diverse microbes than previously considered contribute the N2O emissions in croplands and the main contributors can sequentially change and differ according to the type of fertilizers.

keywords:nitrous oxide,denitrification,nitrification,soil

About the Author
  • Who%20produces%20N2O%20in%20cropland%20fields?

    " target="_blank">
  • Who%20produces%20N2O%20in%20cropland%20fields?

    " target="_blank">
  • Who%20produces%20N2O%20in%20cropland%20fields?

    " target="_blank">
  • Who%20produces%20N2O%20in%20cropland%20fields?

    " target="_blank">
  • google-share

Related Posts

JS1-1:

Rhizosphere is a textbook for microbial N cycle

Posted On 06 10月 2015
, By

JS10-2:

アミノ酸の窒素同位体比からみる食物連鎖

Posted On 06 10月 2015
, By

JS1-3:

Phylogenetic diversity and environmental distribution of denitrifying fungi

Posted On 06 10月 2015
, By

PB-041:

高温性硝化細菌による太陽熱消毒後の土壌硝化活性の回復

Posted On 06 10月 2015
, By

サイト内検索

企業広告

banner
banner

事務局からのお知らせ

Invalid or expired token.

協力団体:日本バイオインフォマティクス学会

banner

協力団体:Japanese Consortium for Human Microbiome

banner

最近の投稿

  • JSME2015ポスター賞
  • 7th JTK Poster Award
  • 第1回JSME・インハイ ポスター賞
  • 土浦大会でのStudent sessionのお知らせ (Student session in 7th JTK)
  • バイオフィルムを構成する細胞外多糖の機能性

アーカイブ

  • 2015年11月
  • 2015年10月
  • 2015年9月
  • 2015年7月
  • 2015年6月
  • 2015年5月
  • 2015年4月
  • 2015年3月
  • 2015年2月
  • 2014年12月
  • 2014年10月
  • 2014年9月
  • 2014年8月
  • 2014年7月
  • 2014年5月
  • 2014年4月
  • 2014年3月
  • 2013年12月
  • 2013年8月
  • 2013年7月

カテゴリー

2025年6月
月 火 水 木 金 土 日
 1
2345678
9101112131415
16171819202122
23242526272829
30  
« 11月    
Copyright (c) Japanese Society of Microbial Ecology. All Rights are Reserved.