Functional classification of uncultured “Ca. Caldiarchaeum subterraneum” using the Maple System
1JAMSTEC, 2Kyushu Institute of Technology, 3National Institute of Basic Biology, 4Mitsubishi Research Institute
The MAPLE system was employed to establish a functional classification of archaeal species and to determine the functionome of the previously uncultivated thermophile “Ca. Caldiarchaeum subterraneum”. Consistent with the results of the phylogenetic analysis, clustering and PCA based on the MCR patterns for all KEGG modules in 79 archaeal species suggested that the overall metabolic and physiological potential of C. subterraneum is similar to that of thaumarchaeotic species. However, it possessed almost no genes in the modules required for nitrification and the HP-HB cycle for carbon fixation. However, it possessed all genes in the modules required for central carbohydrate metabolism, such as glycolysis, pyruvate oxidation, and TCA cycle, as well as multiple sets of sugar and BCAA ABC transporters. These features appear to support the predominantly aerobic character of C. subterraneum, which lives in a subsurface thermophilic microbial mat community with a heterotrophic lifestle.
keywords:MAPLE system,Ca. Caldiarchaeum subterraneum,Functional classification,Archaea,Aigarchaeota