PN-224 (JTK):

Transcriptome Analysis of Agromyces luteolus with Zearalenone-Removal Activity

Zearalenone (ZEA) is a mycotoxin produced by Fusarium spp. and occurs in several cereals. ZEA contaminated cereals is a major threat for food/feedstuff safety. Therefore, ZEA decontamination of cereals is important for food and feedstuff production. We previously isolated and identified Agromyces luteolus M15 from soil as a strain with ZEA removal activity. ZEA removal by the isolate were investigated over time in liquid cultures using high performance liquid chromatography (HPLC). The A. luteolus M15 strain revealed the greater activity for ZEA removal compared to other isolates. Whole genome of M15 strain was analyzed by next-generation sequencing process. Draft genome of M15 revealed 3,792 Mb in genome size with 23 contigs. GC content of M15 genome was 71.5 % which was similar to other phylum Actinobacteria strains. We compared the transcriptome of A. luteolus M15 strain cells with or without ZEA by using RNA sequencing. Transcriptome analysis revealed that 29 genes and 24 genes were up-regulated or down-regulated over two-fold in A. luteolus M15 in the presence of ZEA, respectively. The up-regulated genes included genes encoding regulatory proteins, membrane proteins, several hydrolases, and transporters. Efforts to search for the gene(s) involved in ZEA removal or degradation are in progress.

keywords:Agromyces luteolus,Fusarium,RNAseq,Zearalenone removal