Metagenomic analysis of paddy soil microbial community to reveal methane emission mechanisms
1Faculty of Biotechnology, SARI, Jeju National University,
Large portion of anthropogenic methane is emitted from rice field. Types of fertilizers affect carbon availabilities for those methanogenic microbes, who produce methane in anaerobic condition. Thus irrigation management and types of fertilizers are major driving forces of methane emission in rice fields. Two weeks after transplanting 15-day-old rice seedlings, significantly higher amount of methane emission was observed from rice fertilized with swine manure. Pre-transplanting flooding increased methane emission by 30%. Although these conditions did not affect overall paddy soil microbial communities, fertilizers brought significantly different changes in abundance within minorities, including archaeal methanogenic species and bacterial substrate providers for bio-methane production. Shared species (OTUs) analysis with swine fecal microbial communities indicated the swine manure origin of key methane-producing microbes. Comparative functional metagenome analysis agreed with changes in abundance of methanogenic species and methane metabolism related genes.
keywords:Methane,Microbial community,Paddy,Global warming,Fertilizer