PI-132:

Operational taxonomic units (OTUs)-based analysis of swine gut microbiota in response to the treatment with tylosin, an antibiotic growth promoter (AGP)

Antibiotics have been used extensively as growth promoters (AGPs) in food animal production. However, the use of AGPs in animal feeds was banned in developed countries such as the European Union and South Korea due to problems like antibiotic resistance gene dissemination in the environment and antibiotic residues in meat. Moreover, the mechanism of action for AGPs has not yet been fully understood. The work presented here was to characterize the gut microbiome of pigs, receiving AGP tylosin compared with untreated pigs through operational taxonomic units (OTUs)-based analysis. In this study, we detected significantly different OTUs in between tylosin-untreated pigs and treated pigs, according to growth stages such as 20-40 kg, 40~60 kg, 60~80kg, and 80~100 kg (p=0.05). Two clusters of OTUs were observed to show different OTUs distribution in between control and tylosin group. One was observed in only 80~100 kg (Cluster Ⅰ), and the other was observed in overall growth stage (Cluster Ⅱ). In addition, gut microbiota were observed significantly difference of gut microbiota between control and tylosin pigs in growth stage from 20 to 80 kg according to AMOVA test (p=0.05). Our results showed that tylosin altered the microbial population although weight gain was not significantly different. Here we present Miseq-based gut microbiota analysis to investigate effects of antibiotics.

keywords:antibiotic growth promoter,gut microbiome,operational taxonomic units ,gut microbiota ,Miseq