Identification and characterization of the soil-specific expression genes in Pseudomonas putida
1Institute of Low Temperature Science, Hokkaido Univ.
Characterization of microbial genes that are specifically induced during living is critical to the understanding of the mechanism which microbes survive and persist in soil. Moreover, there are many genes not to be induced in vitro in the bacterial genome. We present a comprehensive proteome analysis of genome-wide gene expression of the bacterium *Pseudomonas putida* F1 in inoculated non-sterile three different types of soils, compare with batch growth in two standard liquid media at the log and stationary phases. Seven genes were identified as soil-specific expressed genes, which were 2 genes and 1 operon consisted of 5 genes. In the genomic information the one is annotated as a hypothetical protein, another one is a nitric oxide dioxygenase, and the function of the operon is unknown. From expression factor analysis, the gene coding for nitric oxide dioxygenase was induced by nitric oxide, 5 genes of the operon were found to be induced under starvation of phosphate. These results indicate that it is possible to know the microenvironment surrounding the bacterial cells in natural environment.